ABSTRACT
Objective To investigate the effect of doxycycline on the proliferation of human small cell lung cancer H446 cells in vitro and its possible mechanisms. Methods H446 cells were treated with different concentrations of doxycycline for 24,48,72 or 96 h. Cell proliferation was measured by CCK-8 assay,IC50 was caculated by Bliss,and cell apoptosis was examined by TUNEL assay. The protein and mRNA expression of Bcl-2 and Bax in H446 cells were detected by Western blot and real time-PCR,respectively. Re-sults Doxycycline inhibited the proliferation of H446 cells in a concentration-time dependent manner. The half inhibitory concentra-tions(IC50)of doxycycline on cell proliferation in H446 cells were approximately 24.10 mg/L for 24 h treatment group,10.98 mg/L for 48 h treatment group,8.20 mg/L for 72 h treatment group and 8.03 mg/L for 96 h treatment group,respectively. Furthermore,doxycy-cline treatment could also induce the apoptosis of H446 cells in a concentration-dependent manner. Moreover,doxycycline treatment dramatically down-regulated the expression of Bcl-2 and up-regulated the expression of Bax in H446 cells both at the protein and at the mRNA levels. The differences were statistically significant(P<0.05). Conclusion Doxycycline can inhibit the proliferation of H446 cells by inducing apoptosis via down-regulating the expression of Bcl-2 and up-regulating the expression of Bax. Doxycycline has great potential as a chemotherapeutic agent for human small cell lung cancer.
ABSTRACT
Objective By selecting the prescriptions of treating blood group incompatibility in modern literature, and exploring their regularity, to establish the overall thought of traditional Chinese medicine in treatment.Methods 90 articles on maternal-fetal blood group incompatibility were found.Statistical methods were adopted to summarize high frequently used medicines and formula. Results High frequently used medicines were Virgate Wormwood Herb, Baical Skullcap Root, Rhubarb, Cape Jasmine Fruit, White peony Alba, Chinese Angelica,Largehead Atractylodes Rhizome,Motherwort Herb,and Milkvetch Root, which were mentioned for 238 times in the literatures, occupying 72.79% of the total frequency. Conclusion Based on this result,heat-clearing and dampness-removing, activating blood circulation to eliminate blood stasis was setup as the principle for treating this disease.
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0.05).CONCLUSION:Domestic octreotide acetate have same effect to imported octreotide acetate in the treatment of Esophageal and gastric Varices Bleeding which is caused by portal hypertension in patients with decompensated liver cirrhosis.Stopping bleeding rate of the achieved the similar level with.